Exercise: Protein And Nucleic Acid Interactions

Questions for: Protein And Nucleic Acid Interactions

Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?
A:
Ile and Val
B:
Lys and Arg
C:
Leu and Ala
D:
Cys and Met
Answer: B
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Cleavage of DNA by EcoRI endonuclease results in the formation of
A:
two 3'-OH ends and two 5'-phosphate ends
B:
one 3'-OH end and one 5'-phosphate end
C:
two 3'-phosphate ends and two 3'-OH ends
D:
one 3'-OH end and two 5'-phosphate end
Answer: A
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The enzyme that joins DNA cuts is called
A:
joinase
B:
ligase
C:
DNA phosphorylase
D:
reverse transcriptase
Answer: B
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The type II restriction endonucleases, bind to DNA sites
A:
using H-bonds for specificity
B:
that are usually palindromic
C:
using non-sequence-dependent backbone interactions
D:
all of the above
Answer: D
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Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
A:
positive direction
B:
3' to 5' direction
C:
5' to 3' direction
D:
negative direction
Answer: C
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