Exercise: Polymerase Chain Reaction

Questions for: Polymerase Chain Reaction

How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?
A:
Two ds DNA molecule
B:
Three ds DNA molecule
C:
Eight ds DNA molecule
D:
Four ds DNA molecule
Answer: D
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A PCR cycle consists of
A:
three steps, denaturation, primer annealing and elongation
B:
three steps, denaturation, initiation and elongation
C:
three steps, primer annealing, elongation and termination
D:
three steps, initiation, elongation and termination
Answer: A
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Restriction maps
A:
allows comparison between DNA molecules without the need to determine nucleotide sequence
B:
allows comparison between DNA molecules but requires to determine nucleotide sequence
C:
does not allow comparison between DNA molecules
D:
none of the above
Answer: A
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Transformation means
A:
formation of a pilus
B:
acquiring DNA from the bacterial cell environment
C:
plasmid containing a F factor
D:
F+ and F- strains of bacteria
Answer: B
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PCR can be used to amplify a specific fragment of DNA from which of the following?
A:
A drop of blood
B:
A hair follicle
C:
A fragment of skin
D:
All of these
Answer: D
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