Exercise: Polymerase Chain Reaction

Questions for: Polymerase Chain Reaction

The template for PCR is
A:
RNA
B:
single stranded DNA
C:
double stranded DNA
D:
none of these
Answer: C
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The cloning step in PCR/sequencing analysis of microbial communities is necessary for
A:
the amplification process
B:
preventing contamination by outside DNA
C:
separating the different rRNA gene sequences in the mixture
D:
none of these
Answer: C
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RNA populations can also be used as PCR templates after reverse transcription into
A:
c DNA
B:
t RNA
C:
m DNA
D:
r RNA
Answer: A
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The original enzyme(s) used in PCR reaction is/are known as
A:
E. coli DNA polymerase
B:
E. coli RNA polymerase
C:
Taq polymerase
D:
all of these
Answer: A
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The efficiency of amplification in PCR in later cycles is reduced due to
A:
reduction in substrate concentration
B:
insufficient enzyme and time to synthesize mass quantity of DNA
C:
build up of PCR product which competes with primers for hybrid formation
D:
all of these
Answer: D
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