Exercise: Media Required For Plant Cell Culture

Questions for: Media Required For Plant Cell Culture

Neutralized activated charcoal is occasionally added to young regenerating cultures to
A:
remove toxic phenolics produced by the stressed plant cell
B:
help to remove plants growth regulators introduced at an earlier stage
C:
both (a) and (b)
D:
maintain the pH of the medium
Answer: C
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What are the macronutrients used in plant cell culture medium?
A:
N, P, K, S, Na
B:
N, P, K, Ca, Cl
C:
N, P, K, S, Ca
D:
N, P, Ca, Na, Cl
Answer: C
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What is 'nurse' or conditioned medium?
A:
It is the media full of growth factors used for the growth of cells
B:
It is the medium added to nurse the callus culture
C:
Both (a) and (b)
D:
It is the liquid medium removed from the suspension of fast growing cells
Answer: D
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Very high sugar concentration (40-100 g/l) have been used
A:
in specialized secondary metabolite production
B:
to adjust the osmotic potential of the media in short term treatment for regeneration
C:
Both (a) and (b)
D:
none of these
Answer: C
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Which of the following is not true about nurse or conditioned medium?
A:
It is liquid removed from the suspension of fast growing cells
B:
It contains uncharacterized growth factor released by growing cells
C:
It is used in the culture of regenerating protoplast
D:
it is removed aseptically from the culture and is autoclaved before use
Answer: D
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