Downstream Processing

Selectivity can be expressed as

A:

ratio of the distribution coefficients in different solute

B:

mass fraction of the solute in the extract phase at equilibrium

C:

mass fraction of the solute in the raffinate phase

D:

ratio of the mass fraction of the solute in the extract phase to the raffinate phase

Answer: A

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Filtration leaf test results indicate that the filtration rate of a protein product is 100 dry lbs/(ft² hr). What size production filter would be required to obtain 100 dry lbs of filter cake/hr?

A:

1.54 ft²

B:

3.08 ft²

C:

65 ft²

D:

65 lbs/ ft².hr

Answer: A

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Assuming the laminar flow across the filter, the rate of filtration (dV_f/dt) can be expressed as a function of pressure drop Dp by the modified D'Arcy's equation as (where A is the area of filtering surface, K is the D'Arcy's filter cake permeability and L is the thickness of the filter cake)

A:

(1/A) dV_f/dt = Δp/μ(L/K)

B:

dV_f/dt = (1/A)Δp/μ(L/K)

C:

(1/A) dV_f/dt = Δp/μ LK

D:

dV_f/dt = (1/A)Δp/μ LK

Answer: A

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The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between

A:

5-15% by volume

B:

15-30% by volume

C:

30-60% by volume

D:

60-90% by volume

Answer: C

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Maintenance cost varies from

A:

0-4% of the capital cost

B:

12-16% of the capital cost

C:

4-8% of the capital cost

D:

8-12% of the capital cost

Answer: C

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Questions for: Downstream Processing