Exercise: Cloning Vectors

Questions for: Cloning Vectors

Difference between λ gt 10 and λ gt 11 vectors is that
A:
λ gt 11 is an expression vector
B:
λ gt 10 is an expression vector
C:
λ gt 10 is a replacement vector
D:
λ gt 11 is a replacement vector
Answer: A
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A plasmid can be considered as a suitable cloning vector if
A:
it can be readily isolated from the cells
B:
it possesses a single restriction site for one or more restriction enzymes
C:
insertion of foreign DNA does not alter its replication properties
D:
All of the above
Answer: D
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pBR 322 has/have which of the following selection marker(s)?
A:
Ampr
B:
Tetr
C:
Both (a) and (b)
D:
Kanr
Answer: C
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Which of the following is not true about phagemid?
A:
Contain functional origin of replication of the plasmid and λ phage
B:
May be propagated as a plasmid or as phage in appropriate strain
C:
Contain λ att site
D:
Can only be propagated as phage
Answer: D
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Maximum size of foreign DNA that can be inserted into a replacement vector is
A:
25-30 kb
B:
18-20 kb
C:
20-25 kb
D:
40-50 kb
Answer: C
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