Exercise: Cloning Vectors

Questions for: Cloning Vectors

Cosmids lack
A:
genes coding for viral proteins
B:
origin of replication
C:
marker genes coding for replication
D:
cleavage site for the insertion of foreign DNA
Answer: A
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Plasmids which are maintained as multiple copy number per cell are known as
A:
stringent plasmids
B:
relaxed plasmids
C:
cryptic plasmids
D:
none of these
Answer: B
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Maximum size of foreign DNA that can be inserted into an insertion vector is
A:
35 kb
B:
18 kb
C:
50 kb
D:
27 kb
Answer: B
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Phagemid consist of
A:
plasmid vector carrying λ phage's cos site
B:
plasmid vector carrying λ attachment (λ att) site
C:
plasmid vector carrying origin of replication of λ phage only
D:
plasmid vector carrying origin of replication of plasmid only
Answer: B
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Cryptic plasmids
A:
do not exhibit any phenotypic trait
B:
exhibit many phenotypic traits
C:
exhibit one phenotypic traits
D:
exhibit antibiotic resistance
Answer: A
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