Exercise: Cloning Vectors

Questions for: Cloning Vectors

Conjugative plasmids
A:
exhibit antibiotic resistance
B:
do not exhibit antibiotic resistance
C:
carry transfer genes called the tra genes
D:
do not carry transfer genes
Answer: C
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Stuffer is
A:
the right arm of the vector DNA
B:
the left arm of the vector DNA
C:
central fragment of the vector DNA
D:
none of the above
Answer: C
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Select the wrong statement about plasmids?
A:
It is extrachromosomal
B:
It is double stranded
C:
Its replication depends upon host cell
D:
It is closed and circular DNA
Answer: C
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λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
A:
6-7 kb
B:
1-2 kb
C:
40-44 kb
D:
20-23 kb
Answer: A
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λ ZAP vector is an example of
A:
phage
B:
phagemid
C:
cosmid
D:
plasmid
Answer: B
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