Cloning Vectors

Inserted DNA in λ gt 11 can be expressed as

A:

β-galactosidase fused protein

B:

free protein in the cytoplasm

C:

free protein that is secreted out

D:

all of the above

Answer: A

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Single stranded vectors are useful

A:

for sequencing of cloned DNA

B:

for oligonucleotide directed mutagenesis

C:

for probe preparation

D:

all of the above

Answer: D

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Phagemid vectors are

A:

combination of plasmid and phage λ

B:

combination of phages and cosmid

C:

phages carrying properties of plasmids

D:

all of the above

Answer: A

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M 13 is an example of

A:

filamentous phage

B:

single stranded DNA vector

C:

both (a) and (b)

D:

plasmid

Answer: C

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Cos site of the cosmids

A:

consists of 12 bases

B:

helps whole genome in circularization and ligation

C:

both (a) and (b)

D:

contains cleavage site

Answer: C

No answer description is available. Let's discuss.

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Questions for: Cloning Vectors