Exercise: Recombinant Dna

Questions for: Recombinant Dna

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
A:
DNA polymerase
B:
Restriction endonuclease
C:
DNA ligase
D:
DNA helicase
Answer: C
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A genomic library is
A:
a database where the sequence of an organism's genome is stored
B:
a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
C:
a book that describes how to isolate DNA from a particular organism
D:
a place where the information of the genetic organization of organisms are kept.
Answer: B
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For gene probes to be useful they must
A:
be large enough to contain gene-specific sequences
B:
be labeled in some manner to allow detection
C:
both (a) and (b)
D:
none of the above
Answer: C
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Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
A:
photoreactivation
B:
gel electrophoresis
C:
autoradiography
D:
blotting
Answer: B
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Which of the following vector can maintain the largest fragment of foreign DNA?
A:
YAC
B:
Cosmid
C:
Plasmid
D:
Phage
Answer: A
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