Immobilized Enzyme

To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as

A:

reaction rate/rate if not slowed by diffusion

B:

rate if not slowed by diffusion/ reaction rate

C:

actual reaction rate/ rate if not slowed by diffusion

D:

rate if not slowed by diffusion/ actual reaction rate

Answer: C

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The covalent attachment of enzyme molecules is via

A:

nonessential amino acids residues to water insoluble, functional supports

B:

essential amino acids residues to water insoluble, functional supports

C:

nonessential amino acids residues to water soluble, functional supports

D:

essential amino acids residues to water soluble, functional supports

Answer: A

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The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

A:

copolymerization or microencapsulation

B:

crosslinking using multifunctional reagents

C:

adsorption

D:

all of the above

Answer: A

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The support material for immobilization of cells of Bacillus subtilis is

A:

ion exchange resins

B:

gelatin

C:

Anthracite

D:

agarose and carbodiimide

Answer: D

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Which of the following is not a physical method of immobilization?

A:

Adsorption

B:

Entrapment

C:

Micro encapsulation

D:

None of these

Answer: D

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Questions for: Immobilized Enzyme